A Computer-assisted Multi-electrode Patch-clamp System
نویسندگان
چکیده
منابع مشابه
A computer-assisted multi-electrode patch-clamp system.
The patch-clamp technique is today the most well-established method for recording electrical activity from individual neurons or their subcellular compartments. Nevertheless, achieving stable recordings, even from individual cells, remains a time-consuming procedure of considerable complexity. Automation of many steps in conjunction with efficient information display can greatly assist experime...
متن کاملMicrofluidic system for planar patch clamp electrode arrays.
We present a microfluidic system integrated with disposable cell interface partitions for simultaneous patch clamp recordings. Glass-supported poly(dimethylsiloxane) (PDMS) partitions, having a 2 microm air-blown aperture, were reversibly sealed to a microfluidic system including PDMS channels with isolation valves and microfabricated Ag/AgCl electrodes. Gigaseal recordings from RBL-1 cells wer...
متن کاملMicrofluidic System for Planar PDMS Patch Clamp Electrode Arrays
Session Title: Micro-and Nanotechnology, Nanopores Presentation Number: 1448-Pos Abstract Title: Microfluidic System for Planar PDMS Patch Clamp Electrode Arrays Location: Halls A/B/C/D Topic: 9G Biotechnology & Bioengineering Author Block: Xiaohui Li, MS, Kathryn G. Klemic, James F. Klemic, Mark A. Reed, Fred J. Sigworth. Yale University, New Haven, CT, USA. Air-molding is an easy way to fabri...
متن کاملSingle-cell Patch-clamp Measurement System
29 THE 4TH ANNUAL IEEE INTERnational Conference on Nano/Microengineered Molecular Systems (IEEE-NEMS) was held at the Sheraton Dameisha Resort in Shenzhen, China, 5–8 January 2009. More than 300 papers were presented by leading micro/nanotechnology researchers in academia and industry from around the globe. Topical areas of the conference included microelectro mechanical systems (MEMS), BioMEMS...
متن کاملMicro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials.
Maintaining a stable electrode potential is critical for patch-clamp measurements. The electrode potential of conventional patch electrode-holder assembly, where an Ag/AgCl wire is in direct contact with the patch pipette filling solution, is subject to drift if the pipette solution contains a low concentration of chloride ions (Cl-). We developed an agar bridge of 3 M KCl filled in a polyimide...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2013
ISSN: 1940-087X
DOI: 10.3791/50630